DanStem: [Florian Hermann] Video 2 - Segmentation of lumens and NGN3pos cells.

Video of segmented immunofluorescent confocal z-stack of d10 N3.6.1 cells in the MGO culture showing the polarisation of endocrine progenitors (NGN3pos NGN3-GFP-expressing). Endocrine progenitors (NGN3 – red; NGN3-GFP - green), apical membranes (EZR - white), and nuclei (DAPI - blue) were stained. Polarised membranes of endocrine progenitors was also shown in blue.